U371-GFP ILtoEK
(Plasmid
#24472)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24472 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET17
- Backbone size w/o insert (bp) 3300
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameUnc104
-
SpeciesC. elegans (nematode)
-
Insert Size (bp)1113
-
MutationContains only the kinesin's motor head fused to GFP (1-371) I362E/L365K
-
Entrez Geneunc-104 (a.k.a. CELE_C52E12.2)
-
Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
U371-GFP ILtoEK was a gift from Ron Vale (Addgene plasmid # 24472 ; http://n2t.net/addgene:24472 ; RRID:Addgene_24472) -
For your References section:
Conversion of Unc104/KIF1A kinesin into a processive motor after dimerization. Tomishige M, Klopfenstein DR, Vale RD. Science. 2002 Sep 27. 297(5590):2263-7. 10.1126/science.1073386 PubMed 12351789