K560CLM T324C
(Plasmid
#24460)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24460 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET17b
- Backbone size w/o insert (bp) 3300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKinesin
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Alt nameK490CLM T324C
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Alt nameKIF5B
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SpeciesH. sapiens (human)
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Insert Size (bp)1680
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MutationC7S/C65A/C168A/C174S/C294A/C330S T324C/C421A
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Entrez GeneKIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
K560CLM T324C was a gift from Ron Vale (Addgene plasmid # 24460 ; http://n2t.net/addgene:24460 ; RRID:Addgene_24460) -
For your References section:
Kinesin walks hand-over-hand. Yildiz A, Tomishige M, Vale RD, Selvin PR. Science. 2004 Jan 30. 303(5658):676-8. 10.1126/science.1093753 PubMed 14684828