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Addgene

KRP85
(Plasmid #24448)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24448 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET17b
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kinesin II
  • Alt name
    KRP85-GFP
  • Species
    Sea urchin
  • Insert Size (bp)
    1500
  • Entrez Gene
    KRP85 (a.k.a. KRP85, SPKRP85)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7, GFP-R
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Thr-Ser linker between the KRP and GFP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KRP85 was a gift from Ron Vale (Addgene plasmid # 24448 ; http://n2t.net/addgene:24448 ; RRID:Addgene_24448)

  • For your References section:

    Single-molecule behavior of monomeric and heteromeric kinesins. Pierce DW, Hom-Booher N, Otsuka AJ, Vale RD. Biochemistry. 1999 Apr 27. 38(17):5412-21. 10.1021/bi9830009 PubMed 10220328
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