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Addgene

K420CLM RP
(Plasmid #24443)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24443 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET17b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kinesin
  • Alt name
    K420CLM RP
  • Alt name
    KIF5B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1260
  • Mutation
    C7S/C65A/C168A/C174S/C294A/C330S; aa 1-420 only
  • Entrez Gene
    KIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    K420CLM RP was a gift from Ron Vale (Addgene plasmid # 24443 ; http://n2t.net/addgene:24443 ; RRID:Addgene_24443)

  • For your References section:

    Controlling kinesin by reversible disulfide cross-linking. Identifying the motility-producing conformational change. Tomishige M, Vale RD. J Cell Biol. 2000 Nov 27. 151(5):1081-92. 10.1083/jcb.151.5.1081 PubMed 11086009
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