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Addgene

K222C
(Plasmid #24438)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24438 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET17b
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kinesin
  • Alt name
    K560CLM-GFP
  • Alt name
    KIF5B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1680
  • Mutation
    C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C
  • Entrez Gene
    KIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)
  • Tags / Fusion Proteins
    • GFP (C terminal on insert)
    • 6XHis (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    K222C was a gift from Ron Vale (Addgene plasmid # 24438 ; http://n2t.net/addgene:24438 ; RRID:Addgene_24438)

  • For your References section:

    Controlling kinesin by reversible disulfide cross-linking. Identifying the motility-producing conformational change. Tomishige M, Vale RD. J Cell Biol. 2000 Nov 27. 151(5):1081-92. 10.1083/jcb.151.5.1081 PubMed 11086009
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