RP hk339-GFP
(Plasmid #24431)

• Depositing Lab: Ron Vale
• Publication: Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92.

Backbone

• Vector backbone: pET17b
• Backbone size w/o insert (bp): 3300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kinesin WT
• Alt name: K339-GFP
• Alt name: KIF5B
• Species: H. sapiens (human)
• Insert Size (bp): 1017
• Mutation: monomeric kinesin
• Entrez Gene: KIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)
• Tags / Fusion Proteins:
  • GFP (C terminal on insert)
  • 6XHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7, GFP-R
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry