f2H ∂HD (272)
(Plasmid
#24428)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24428 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSecTag2
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSULF2
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SpeciesH. sapiens (human)
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Insert Size (bp)2300
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MutationAA 418-716 deleted, 23AA N-terminal truncation
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Entrez GeneSULF2 (a.k.a. HSULF-2)
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Tags
/ Fusion Proteins
- FLAG (N terminal on insert)
- Myc (C terminal on insert)
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer CMV-F, T7
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
f2H ∂HD (272) was a gift from Steven Rosen (Addgene plasmid # 24428 ; http://n2t.net/addgene:24428 ; RRID:Addgene_24428) -
For your References section:
Sulf-2, a proangiogenic heparan sulfate endosulfatase, is upregulated in breast cancer. Morimoto-Tomita M, Uchimura K, Bistrup A, Lum DH, Egeblad M, Boudreau N, Werb Z, Rosen SD. Neoplasia. 2005 Nov . 7(11):1001-10. 10.1593/neo.05496 PubMed 16331886