PCLP-2/NT-IgG (104)
(Plasmid #24423)

• Depositing Lab: Steven Rosen
• Publication: Sassetti et al J Biol Chem. 2000 Mar 24. 275(12):9001-10.

Backbone

• Vector backbone: Ig/pEG-BOS
• Backbone size w/o insert (bp): 7300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PCLP-2
• Alt name: Endoglycan
• Species: H. sapiens (human)
• Insert Size (bp): 630
• Mutation: N-terminal (acidic) domain of PCLP-2 (1-630 bp) fused to Human IgG1 Fc
• Entrez Gene: PODXL2 (a.k.a. PODLX2)
• Tag / Fusion Protein:
  • Human IgG1 Fc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI/NheI (destroyed during cloning)
• 3′ cloning site: BamHI/BclI (destroyed during cloning)
• 5′ sequencing primer: EF-1a Forward

Resource Information

• Supplemental Documents:
  • pEF-BOS/IgG information

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PCR from PCLP-2 clone G11H9-11b with primers (CS36 and CS38). Insert had NheI and BclI sites compatible with XbaI and BamHI in vector. Can NOT be excised.

The IgG1 tag was not confirmed in sequencing, but the lab has tested it functionally.