PCLP-2/pCMV (100)
(Plasmid
#24421)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24421 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV6-XL3
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Backbone manufacturerOrigene
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePCLP-2
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Alt nameEndoglycan
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SpeciesH. sapiens (human)
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Insert Size (bp)2265
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Entrez GenePODXL2 (a.k.a. PODLX2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full length PCLP-2 clone isolated from Origens fetal Brain Library clone #G11H9-11b
A S207F mutation in the gene has no known effect on function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PCLP-2/pCMV (100) was a gift from Steven Rosen (Addgene plasmid # 24421 ; http://n2t.net/addgene:24421 ; RRID:Addgene_24421) -
For your References section:
Identification of endoglycan, a member of the CD34/podocalyxin family of sialomucins. Sassetti C, Van Zante A, Rosen SD. J Biol Chem. 2000 Mar 24. 275(12):9001-10. 10.1074/jbc.275.12.9001 PubMed 10722749