Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2
(Plasmid #24419)

• Depositing Lab: Edward Hsiao
• Publication: Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Backbone

• Vector backbone: pRosa26
• Backbone manufacturer: Plasmid 21714: pROSA26-1 (Soriano Lab)
• Backbone size w/o insert (bp): 4486
• Vector type: Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RS1
• Alt name: Exp-R26(EF1α-tTA/TetO-mCh-Rs1)
• Species: H. sapiens (human)
• Insert Size (bp): 1166
• Mutation: Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence
• Entrez Gene: HTR4 (a.k.a. 5-HT4, 5-HT4R)
• Tags / Fusion Proteins:
  • mCherry (N terminal on backbone)
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: DsRed1-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Rosa26 targeting vector containing the EF1α-tTA/TetO-mCh-Rs1 cassette flanked by insulator sequences