pRosa26 R1-ccdB-R2 RexNeo PI-SceI
(Plasmid #24418)

• Depositing Lab: Edward Hsiao
• Publication: Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Backbone

• Vector backbone: pRosa26-1
• Backbone manufacturer: Soriano Lab (Addgene Plasmid# 21714)
• Backbone size w/o insert (bp): 9846
• Vector type: Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: ccdB cells
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Alt name: pR26 R1R2 RexNeo PI-SceI
• Insert Size (bp): 3788

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • Ed's Primer Suggestions

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Murine Rosa26 targeting vector with R1R2 Gateway sites. Includes a ccdB cassette, Rex-Neo selection marker cassette, and DTA selection cassette.

Plasmid was constructed as follows: The pRosa26-1 plasmid (Addgene plasmid number 21714) was digested at the XbaI cloning site and a PCR product containing the attR1-ccdb-attR2 sequences from pDest27 and an additional PacI site was introduced. The Rex-Neo resistance cassette from the αMHC-eGFP-Rex-Neo lentivirus plasmid was PCR-cloned into the PacI site to allow for antibiotic selection in eukaryotic cells with G418