pAdEasy-EGFP-Tubulin
(Plasmid
#24411)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24411 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAdEasy-1
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Backbone manufacturerStratagene, ATCC
- Backbone size w/o insert (bp) 33414
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Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTubulin
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Alt nameTUBA1B
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SpeciesH. sapiens (human)
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Insert Size (bp)1600
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Entrez GeneTUBA1B (a.k.a. K-ALPHA-1)
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F, EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
EGFP-Tubulin was cloned into pShuttle-CMV between KpnI and NotI then recombined into pAdEasy-1
AdEasy is a registered trademark of the Johns Hopkins University.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAdEasy-EGFP-Tubulin was a gift from Torsten Wittmann (Addgene plasmid # 24411 ; http://n2t.net/addgene:24411 ; RRID:Addgene_24411) -
For your References section:
GSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. Kumar P, Lyle KS, Gierke S, Matov A, Danuser G, Wittmann T. J Cell Biol. 2009 Mar 23. 184(6):895-908. 10.1083/jcb.200901042 PubMed 19289791