pEGFP-N1-Barkor
(Plasmid #24402)

• Depositing Lab: Qing Zhong
• Publication: Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4733
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Barkor
• Alt name: Atg14, Atg14L
• Species: H. sapiens (human)
• Insert Size (bp): 1449
• Entrez Gene: ATG14 (a.k.a. ATG14L, BARKOR, KIAA0831)
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry