pcDNA3-Barkor 1-91
(Plasmid #24395)

• Depositing Lab: Qing Zhong
• Publication: Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6.

Backbone

• Vector backbone: pCDNA3.0/Myc/His
• Backbone size w/o insert (bp): 5489
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Barkor
• Alt name: Atg14, Atg14L
• Species: H. sapiens (human)
• Insert Size (bp): 273
• Mutation: Contains amino acids 1-91.
• Entrez Gene: ATG14 (a.k.a. ATG14L, BARKOR, KIAA0831)
• Tags / Fusion Proteins:
  • Myc (C terminal on insert)
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV, T7
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry