pcDNA4-Beclin1 243-450
(Plasmid #24392)

• Depositing Lab: Qing Zhong
• Publication: Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6.

Backbone

• Vector backbone: pCDNA4/TO-3*flag
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Beclin1
• Species: H. sapiens (human)
• Insert Size (bp): 624
• Mutation: Contains amino acids 243-450.
• Entrez Gene: BECN1 (a.k.a. ATG6, VPS30, beclin1)
• Tags / Fusion Proteins:
  • FLAG (C terminal on insert)
  • FLAG (C terminal on insert)
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry