pHL32 (in cHNL135)
(Plasmid
#24387)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24387 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHL32
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
cHNL135: T7 Polymerase is integrated into the chromosome. pHL32 is the reporter plasmid, which contains GFP, driven by a T7 promoter.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHL32 (in cHNL135) was a gift from Han Lim (Addgene plasmid # 24387 ; http://n2t.net/addgene:24387 ; RRID:Addgene_24387) -
For your References section:
A multistep epigenetic switch enables the stable inheritance of DNA methylation states. Lim HN, van Oudenaarden A. Nat Genet. 2007 Feb . 39(2):269-75. 10.1038/ng1956 PubMed 17220888