pcDNA3.1 Neo(+) hSUMF1
(Plasmid #24386)

• Depositing Lab: Carolyn Bertozzi
• Publication: Wu et al Proc Natl Acad Sci U S A. 2009 Mar 3. 106(9):3000-5.

Backbone

• Vector backbone: pcDNA3.1 (+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hSUMF1
• Alt name: human formylglycine generating enzyme
• Alt name: pcDNA3.1-hFGE
• Species: H. sapiens (human)
• Insert Size (bp): 1041
• Entrez Gene: SUMF1 (a.k.a. AAPA3037, FGE, UNQ3037)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry