pQF-ET
(Plasmid
#24365)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24365 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGalW
- Backbone size w/o insert (bp) 11000
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameQF
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SpeciesNeurospora crassa
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Insert Size (bp)2500
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Reference
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The QA1F-SV40polyA fragment was excised from pQF-M2ET,w+ by digestion with EcoRI and
BamHI, blunted by Klenow and inserted into blunted pGalW (Brand and Perrimon, 1993) from which the GAL4-hsp70polyA fragment had been excised by NotI digestion.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQF-ET was a gift from Liqun Luo (Addgene plasmid # 24365 ; http://n2t.net/addgene:24365 ; RRID:Addgene_24365) -
For your References section:
The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990
Map uploaded by the depositor.
