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pQUAST-shibire (ts1)
(Plasmid #24362)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24362 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQUAST
  • Backbone size w/o insert (bp) 9000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Shibire (ts1)
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2500
  • Entrez Gene
    shi (a.k.a. Dmel_CG18102, CG18102, DNM3, DYN, Ddyn, Ddyn3, Ddyn4, Dmel\CG18102, Dyn, Dynamin, Shi, Shibire, Vps1, anon-WO0153538.12, anon-WO0153538.13, anon-WO0153538.14, dDyn, dyn, dynamin, l(1)VII, l(1)shi, shib, shibere, shu)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer pCasper-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Shibire was PCR amplified from genomic DNA of UAS-shibire transgenic flies (Kitamoto, 2001) using genUASFOR and genUASREVsv40 oligos, and ligated into the NotI/KpnI sites of pQUAST.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQUAST-shibire (ts1) was a gift from Liqun Luo (Addgene plasmid # 24362 ; http://n2t.net/addgene:24362 ; RRID:Addgene_24362)

  • For your References section:

    The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990
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