ptubP-QS
(Plasmid #24352)

• Depositing Lab: Liqun Luo
• Publication: Potter et al Volume 141, Issue 3, 536-548, 30 April 2010

Backbone

• Vector backbone: pCasper-tubulin-GAL80
• Backbone size w/o insert (bp): 10000
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: QS
• Species: Neurospora crassa
• Insert Size (bp): 2500
• Tag / Fusion Protein:
  • white gene (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Acc65I (destroyed during cloning)
• 3′ cloning site: NotI (destroyed during cloning)
• 5′ sequencing primer: pCasper-F

Resource Information

• Reference:
  • ptubP-QS (GenBank)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The QS cDNA was excised from pAC-QS with Acc65I and NotI, blunted, and ligated into
a blunted pCasper-tubulin-GAL80, from which the GAL80 insert was removed by digestion with
NotI/XbaI.

There is a ~150bp deletion in the SV40 tail compared to the pUAST-SV40 sequence from Flybase.