pAC-hRluc
(Plasmid #24348)

• Depositing Lab: Liqun Luo
• Publication: Potter et al Volume 141, Issue 3, 536-548, 30 April 2010

Backbone

• Vector backbone: pPac5c-PL
• Backbone size w/o insert (bp): 6500
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hRluc
• Alt name: Synthetic renilla luciferase
• Species: Synthetic
• Insert Size (bp): 900

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Acc65I (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: AC5

Resource Information

• Reference:
  • pAC-hRluc (GenBank)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Synthetic Renilla luciferase that was codon optimized for expression in mammalian
cells (hRluc) was amplified using primers PR444
(ataaGGTACCaaaATGGCTTCCAAGGTGTACGA) and PR443
(ataaGCGGCCGCTTACTGCTCGTTCTTCAGCA), and pGL4.75 (hRluc/CMV, Promega, catalog #
E6931) as the template. The PCR product was cloned into pPAC5C-PL using Acc65I and NotI.