pUAS-GG
(Plasmid #24342)

• Purpose: (Empty Backbone)
• Depositing Lab: Liqun Luo
• Publication: Potter et al Volume 141, Issue 3, 536-548, 30 April 2010

Backbone

• Vector backbone: pBluescript SK(+)
• Backbone size (bp): 3000
• Modifications to backbone: GFP split with an intron (GG)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • 5XGAL4 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BglII (unknown if destroyed)
• 5′ sequencing primer: GFP-N

Resource Information

• Reference:
  • pUAS-GG (GenBank)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The BamHI/BglI fragment from pUAST (Brand and Perrimon, 1993), containing 5 copies
of a GAL4 binding site and the Drosophila hsp70 minimal promoter was subcloned into BamHI site
of pBluescript containing an optimized GFP split with an intron (GG) (Zong et al., 2005). This
strategy generates a hybrid BglII/BamHI site in front of the GFP.