pQUAS-GG
(Plasmid #24336)

• Depositing Lab: Liqun Luo
• Publication: Potter et al Volume 141, Issue 3, 536-548, 30 April 2010

Backbone

• Vector backbone: pBluescript SK(+)
• Backbone size w/o insert (bp): 3000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 5xQUAS/GFP split with an intron (GG)
• Species: Neurospora crassa/other
• Insert Size (bp): 1500

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Reference:
  • pQUAS-GG (GenBank)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains 5 copies of naturally occurring QF binding sites (each 16 bp
long, shown in capital letters, with spacer sequences in small letters):
GGGTAATCGCTTATCCtcGGATAAACAATTATCCtcacGGGTAATCGCTTATCCgctcGGGTAATCG
CTTATCCtcGGGTAATCGCTTATCCtt
This sequence was assembled from overlapping primers and cloned using XhoI and HindIII into
pBluescript containing hsp70 minimal promoter (Brand and Perrimon, 1993) and an optimized GFP
split with an intron (GG) (Zong et al., 2005)