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Addgene

pEAK-Flag/INPP4B
(Plasmid #24324)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24324 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEAK
  • Backbone size w/o insert (bp) 6164
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Inositol Polyphosphate 4-Phosphatase Type II
  • Alt name
    INPP4B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2775
  • Mutation
    none
  • GenBank ID
    BC110918
  • Entrez Gene
    INPP4B
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEAK-Flag/INPP4B was a gift from Lewis Cantley (Addgene plasmid # 24324 ; http://n2t.net/addgene:24324 ; RRID:Addgene_24324)

  • For your References section:

    Evidence that inositol polyphosphate 4-phosphatase type II is a tumor suppressor that inhibits PI3K signaling. Gewinner C, Wang ZC, Richardson A, Teruya-Feldstein J, Etemadmoghadam D, Bowtell D, Barretina J, Lin WM, Rameh L, Salmena L, Pandolfi PP, Cantley LC. Cancer Cell. 2009 Aug 4. 16(2):115-25. 10.1016/j.ccr.2009.06.006 PubMed 19647222
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