pGEX-GST-AGO3
(Plasmid #24318)

• Depositing Lab: Carl Novina
• Publication: Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1259-66.

Backbone

• Vector backbone: pGEX-4T-1
• Backbone size w/o insert (bp): 4969
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AGO3
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• Entrez Gene: AGO3 (a.k.a. EIF2C3)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • Additional Annotated sequence from Novina lab (AGO3)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry