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Addgene

pGEX-GST-AGO2
(Plasmid #24317)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24317 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone size w/o insert (bp) 4969
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human AGO2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • Entrez Gene
    AGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI* (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX-5
  • 3′ sequencing primer pGEX-3
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Note that though the depositor lists XhoI as the 5' cloning enzyme, the AGO2 gene has an internal XhoI site. Addgene's quality control sequence shows that there is no intact XhoI site at the 5' end of the insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-GST-AGO2 was a gift from Carl Novina (Addgene plasmid # 24317 ; http://n2t.net/addgene:24317 ; RRID:Addgene_24317)
  • For your References section:

    Distinct passenger strand and mRNA cleavage activities of human Argonaute proteins. Wang B, Li S, Qi HH, Chowdhury D, Shi Y, Novina CD. Nat Struct Mol Biol. 2009 Dec . 16(12):1259-66. 10.1038/nsmb.1712 PubMed 19946268