pGEX-GST-AGO2
(Plasmid #24317)

• Depositing Lab: Carl Novina
• Publication: Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1259-66.

Backbone

• Vector backbone: pGEX-4T-1
• Backbone size w/o insert (bp): 4969
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: human AGO2
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• Entrez Gene: AGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI* (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pGEX-5
• 3′ sequencing primer: pGEX-3

Resource Information

• Supplemental Documents:
  • Additional Annotated sequence from Novina lab (AGO2)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*Note that though the depositor lists XhoI as the 5' cloning enzyme, the AGO2 gene has an internal XhoI site. Addgene's quality control sequence shows that there is no intact XhoI site at the 5' end of the insert.