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Addgene

7TG
(Plasmid #24314)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24314 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRLSIN.cPPT.PGK-GFP.WPRE
  • Backbone manufacturer
    Naldini/Trono (Addgene #12252)
  • Backbone size w/o insert (bp) 6132
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    7xTcf-eGFP
  • Alt name
    eGFP
  • Alt name
    enhanced GFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    997
  • Mutation
    EGFP is GFP with S65T and F64L mutations

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GGCCCGAAGGAATAGAAG
  • 3′ sequencing primer AACGGGCCACAACTCCTC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The 7xTcf promoter is derived from pSuperTOPflash, published in Veeman MT et al., Curr Biol 13: 680–685 (2003)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TFC repeat sequence is AGATCAAAGGgg

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    7TG was a gift from Roel Nusse (Addgene plasmid # 24314 ; http://n2t.net/addgene:24314 ; RRID:Addgene_24314)

  • For your References section:

    Wnt signaling mediates regional specification in the vertebrate face. Brugmann SA, Goodnough LH, Gregorieff A, Leucht P, ten Berge D, Fuerer C, Clevers H, Nusse R, Helms JA. Development. 2007 Sep . 134(18):3283-95. 10.1242/dev.005132 PubMed 17699607
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