E[beta]P
(Plasmid #24313)

• Depositing Lab: Roel Nusse
• Publication: Fuerer et al PLoS One. 2010 . 5(2):e9370.

Backbone

• Vector backbone: pRRLSIN.cPPT.PGK-GFP.WPRE
• Backbone manufacturer: Naldini/Trono (Addgene #12252)
• Backbone size w/o insert (bp): 6074
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EF1alpha-betacatenin(deltaGSK) // SV40-PuroR
• Alt name: betacat
• Alt name: beta-catenin
• Alt name: puromycin N-acetyltransferase
• Species: M. musculus (mouse)
• Insert Size (bp): 4718
• Mutation: Sites phosphorylated by GSK-3beta have been mutated. S33A, S37A, T41A, S45A
• GenBank ID: NM_007614
• Entrez Gene: Ctnnb1 (a.k.a. Bfc, Catnb, Mesc)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (vec) and AvaII (insert) (destroyed during cloning)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GGCCCGAAGGAATAGAAG
• 3′ sequencing primer: AACGGGCCACAACTCCTC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Beta-catenin(∆GSK) was obtained from Amanda Mikels)
• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid requires a 2nd generation lentiviral packaging system.