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Addgene

E[beta]C
(Plasmid #24312)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24312 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRRLSIN.cPPT.PGK-GFP.WPRE
  • Backbone manufacturer
    Naldini/Trono (Addgene #12252)
  • Backbone size w/o insert (bp) 6074
  • Vector type
    Lentiviral
  • Selectable markers
    mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EF1alpha-betacatenin(deltaGSK) // SV40-mCherry
  • Alt name
    Ctnnb1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4773
  • Mutation
    Sites phosphorylated by GSK-3beta have been mutated. S33A, S37A, T41A, S45A
  • GenBank ID
    NM_007614
  • Entrez Gene
    Ctnnb1 (a.k.a. Bfc, Catnb, Mesc)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (vec) and AvaII (insert) (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GGCCCGAAGGAATAGAAG
  • 3′ sequencing primer AACGGGCCACAACTCCTC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Beta-catenin(∆GSK) was obtained from Amanda Mikels)
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid requires a 2nd generation lentiviral packaging system.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E[beta]C was a gift from Roel Nusse (Addgene plasmid # 24312 ; http://n2t.net/addgene:24312 ; RRID:Addgene_24312)
  • For your References section:

    Lentiviral vectors to probe and manipulate the wnt signaling pathway. Fuerer C, Nusse R. PLoS One. 2010 . 5(2):e9370. 10.1371/journal.pone.0009370 PubMed 20186325