-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 24312 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepRRLSIN.cPPT.PGK-GFP.WPRE
-
Backbone manufacturerNaldini/Trono (Addgene #12252)
- Backbone size w/o insert (bp) 6074
-
Vector typeLentiviral
-
Selectable markersmCherry
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Stbl3
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameEF1alpha-betacatenin(deltaGSK) // SV40-mCherry
-
Alt nameCtnnb1
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)4773
-
MutationSites phosphorylated by GSK-3beta have been mutated. S33A, S37A, T41A, S45A
-
GenBank IDNM_007614
-
Entrez GeneCtnnb1 (a.k.a. Bfc, Catnb, Mesc)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (vec) and AvaII (insert) (destroyed during cloning)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer GGCCCGAAGGAATAGAAG
- 3′ sequencing primer AACGGGCCACAACTCCTC (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byBeta-catenin(∆GSK) was obtained from Amanda Mikels)
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid requires a 2nd generation lentiviral packaging system.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
E[beta]C was a gift from Roel Nusse (Addgene plasmid # 24312 ; http://n2t.net/addgene:24312 ; RRID:Addgene_24312) -
For your References section:
Lentiviral vectors to probe and manipulate the wnt signaling pathway. Fuerer C, Nusse R. PLoS One. 2010 . 5(2):e9370. 10.1371/journal.pone.0009370 PubMed 20186325