EdTP
(Plasmid #24311)

• Depositing Lab: Roel Nusse
• Publication: Fuerer et al PLoS One. 2010 . 5(2):e9370.

Backbone

• Vector backbone: pRRLSIN.cPPT.PGK-GFP.WPRE
• Backbone manufacturer: Naldini/Trono (Addgene #12252)
• Backbone size w/o insert (bp): 6074
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EF1alpha-dnhTcf4 // SV40-PuroR
• Alt name: Tcf4
• Alt name: dominant negative tcf4
• Alt name: puromycin N-acetyltransferase
• Species: H. sapiens (human)
• Insert Size (bp): 4114
• Mutation: 31 first amino acids of hTcf4 have been deleted.
• GenBank ID: NM_030756
• Entrez Gene: TCF7L2 (a.k.a. TCF-4, TCF4)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (vec) and AvaII (insert) (destroyed during cloning)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GGCCCGAAGGAATAGAAG
• 3′ sequencing primer: AACGGGCCACAACTCCTC

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: The dominant-negative, flag tagged Tcf4 was obtained from Amanda Mikels)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid requires a 2nd generation lentiviral packaging system.