7TGP
(Plasmid #24305)

• Depositing Lab: Roel Nusse
• Publication: Fuerer et al PLoS One. 2010 . 5(2):e9370.

Backbone

• Vector backbone: pRRLSIN.cPPT.PGK-GFP.WPRE
• Backbone manufacturer: Naldini/Trono (Addgene #12252)
• Backbone size w/o insert (bp): 6132
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Due to potential for recombination, Addgene recommends screening up to 10 colonies by restriction digest prior to storing the plasmid.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 7xTcf-eGFP // SV40-PuroR
• Alt name: GFP
• Alt name: puromycin N-acetyltransferase
• Alt name: enhanced GFP
• Species: Aequorea victoria (modified) / Streptomyces alboniger
• Insert Size (bp): 2094
• Mutation: EGFP is GFP with S65T and F64L mutations

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GGCCCGAAGGAATAGAAG
• 3′ sequencing primer: AACGGGCCACAACTCCTC

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: The 7xTcf promoter is derived from pSuperTOPflash, published in Veeman MT et al., Curr Biol 13: 680–685 (2003)
• Articles Citing this Plasmid:
  • 31 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

TCF repeat sequence is AGATCAAAGGgg.

This plasmid requires a 2nd generation lentiviral packaging system.