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Addgene

7TGC Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb extend DNA ladder and a digestion of plasmid 24304 with NheI-HF and SalI-HF.

We observed the expected pattern of bands:

NheI-HF single digest: Linearized plasmid band ~8.2kb

NheI-HF / SalI-HF double digest: Bands at 6 and 2kb