pME18s-HA-hFIP200
(Plasmid #24303)

• Depositing Lab: Noboru Mizushima
• Publication: Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Backbone

• Vector backbone: pME18s
• Backbone size w/o insert (bp): 3410
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FIP200
• Alt name: focal adhesion kinase family interacting protein of 200 kD
• Alt name: RB1CC1
• Alt name: Rb1-inducible coiled coil protein 1
• Species: H. sapiens (human)
• Insert Size (bp): 4785
• GenBank ID: BC017556
• Entrez Gene: RB1CC1 (a.k.a. ATG17, CC1, FIP200, PPP1R131)
• Tag / Fusion Protein:
  • 3xHA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry