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p3xFLAG-CMV10-mApg16L
(Plasmid #24302)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24302 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3xFLAG-CMV-10
  • Backbone manufacturer
    sigma-aldrich
  • Backbone size w/o insert (bp) 6299
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    autophagy related genes 16L
  • Alt name
    Atg16L
  • Species
    M. musculus (mouse)
  • Mutation
    V583M
  • GenBank ID
    AI037166 NM_001205392
  • Entrez Gene
    Atg16l1 (a.k.a. 1500009K01Rik, Apg16l, Atg16l, WDR30)
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV 30 sequencing primer
  • 3′ sequencing primer CMV 24 sequencing primer
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    IMAGE consortium clone 1480862
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-CMV10-mApg16L was a gift from Noboru Mizushima (Addgene plasmid # 24302 ; http://n2t.net/addgene:24302 ; RRID:Addgene_24302)

  • For your References section:

    Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate. Mizushima N, Kuma A, Kobayashi Y, Yamamoto A, Matsubae M, Takao T, Natsume T, Ohsumi Y, Yoshimori T. J Cell Sci. 2003 May 1. 116(Pt 9):1679-88. 10.1242/jcs.00381 PubMed 12665549
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