pMT-Diaphanous-GFP
(Plasmid #24287)

• Depositing Lab: Ron Vale
• Publication: Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

Backbone

• Vector backbone: pMT-WG
• Backbone size w/o insert (bp): 6786
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Diaphanous
• Alt name: CG1768
• Alt name: Dia
• Species: D. melanogaster (fly)
• Insert Size (bp): 3276
• GenBank ID: NM_165341
• Entrez Gene: dia (a.k.a. Dmel_CG1768, 38E.16, CG1768, DIA, Dia, Diaphanous, Dias, Dmel\CG1768, l(2)k07135, ms(2)04138)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AttR1 (unknown if destroyed)
• 3′ cloning site: AttR2 (unknown if destroyed)
• 5′ sequencing primer: M13 Fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: I received the cDNA from the lab of Mark Peifer at UNC. I amplified the cDNA and inserted it into the pENTR D Topo vector and then ligated it into the pMT-WG vector using LR cloning.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry