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pMT-Pav-GFP
(Plasmid #24286)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24286 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT-GFP
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4200
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pavarotti
  • Alt name
    MKLP1
  • Alt name
    Kinesin-6
  • Alt name
    CG1258
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2664
  • GenBank ID
    NM_057677
  • Entrez Gene
    pav (a.k.a. Dmel_CG1258, 0934/13, 0973/08, 2A12, 2A12MEL, CG1258, DmKLP1/pav, DmMKLP1, DmPAV, DmPav, Dmel\CG1258, KIF 23, KIF23, MKLP1, Mklp1, PAV, PAV-KLP, PAV-MKLP, PAV/KLP, Pav, Pav KLP, Pav-KLP, Pav-Klp, Pav-klp, PavKLP, anon-EST:fe2A12, anon2A12, group D, kinesin-6, pav-KLP, pav-Klp)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was originally generated by Dr. Gohta Goshima and published in MBOC. Vol. 16, 3896–3907.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-Pav-GFP was a gift from Ron Vale (Addgene plasmid # 24286 ; http://n2t.net/addgene:24286 ; RRID:Addgene_24286)

  • For your References section:

    Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells. Vale RD, Spudich JA, Griffis ER. J Cell Biol. 2009 Sep 7. 186(5):727-38. 10.1083/jcb.200902083 PubMed 19720876
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