pMT-Aurora B-GFP
(Plasmid #24285)

• Depositing Lab: Ron Vale
• Publication: Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

Backbone

• Vector backbone: pMT-WG
• Backbone size w/o insert (bp): 6786
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Aurora B Kinase
• Alt name: CG6620
• Alt name: iAL
• Alt name: IplI-aurora-like kinase
• Species: D. melanogaster (fly)
• Insert Size (bp): 1180
• GenBank ID: NM_057988
• Entrez Gene: aurB (a.k.a. Dmel_CG6620, ABK, Aur B, AurB, Aurora B, Aurora-B, CG6620, DmAIRK2, DmAurB, DmAuroraB, Dmel\CG6620, IAL, IAL1, Ial, ial, ial/AurB)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AttR1 (unknown if destroyed)
• 3′ cloning site: AttR2 (unknown if destroyed)
• 5′ sequencing primer: M13 Fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: I obtained this plasmid from Dr. Gilles Hickson in the laboratory of Dr. Pat O'Farrell.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry