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Addgene

pMT-Mad2-GFP
(Plasmid #24284)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24284 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT-WG
  • Backbone size w/o insert (bp) 6786
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mad2
  • Alt name
    CG17498
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    624
  • GenBank ID
    NM_139734
  • Entrez Gene
    mad2 (a.k.a. Dmel_CG17498, CG17498, Dmel\CG17498, MAD2, Mad2)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AttR1 (unknown if destroyed)
  • 3′ cloning site AttR2 (unknown if destroyed)
  • 5′ sequencing primer M13 Fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This cDNA was amplified from a library, ligated into pENTR-D-Topo and then the pENTR insert was ligated into pMT-WG.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-Mad2-GFP was a gift from Ron Vale (Addgene plasmid # 24284 ; http://n2t.net/addgene:24284 ; RRID:Addgene_24284)

  • For your References section:

    Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore. Griffis ER, Stuurman N, Vale RD. J Cell Biol. 2007 Jun 18. 177(6):1005-15. 10.1083/jcb.200702062 PubMed 17576797
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