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Addgene

pMT-GFP-p150Dynactin
(Plasmid #24283)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24283 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT-GW
  • Backbone manufacturer
    Drosophila Gateway Vector Collection
  • Backbone size w/o insert (bp) 5943
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p150 Dynactin
  • Alt name
    Glued
  • Alt name
    CG9206
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    3795
  • GenBank ID
    NM_079337
  • Entrez Gene
    DCTN1-p150 (a.k.a. Dmel_CG9206, CG9206, DCTN1, DYNA_DROME, Dmel\CG9206, FCP-D, Gl, Glu, Glued, P150, P150A, P150[Glued], gl, p150, p150/Glued, p150/glued, p150[Glued], p150[glued], t1)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site AttR1 (unknown if destroyed)
  • 3′ cloning site AttR2 (unknown if destroyed)
  • 5′ sequencing primer pMT-Fwd
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gohta Goshima

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Dr. Goshima made a C-terminal GFP p150 plasmid and Vale lab used his pENTR vector to make an N-terminal fusion using Gateway cloning.

Y1238H mutation detected in Addgene's BGH-rev sequence is not likely to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-GFP-p150Dynactin was a gift from Ron Vale (Addgene plasmid # 24283 ; http://n2t.net/addgene:24283 ; RRID:Addgene_24283)

  • For your References section:

    Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore. Griffis ER, Stuurman N, Vale RD. J Cell Biol. 2007 Jun 18. 177(6):1005-15. 10.1083/jcb.200702062 PubMed 17576797
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