pMT-GFP-Spindly
(Plasmid
#24282)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24282 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMT-GW
- Backbone size w/o insert (bp) 5943
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpindly
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Alt nameCG15415
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SpeciesD. melanogaster (fly)
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Insert Size (bp)2343
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GenBank IDNM_134947
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Entrez GeneSpindly (a.k.a. Dmel_CG15415, CG15415, Dmel\CG15415)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AttR1 (unknown if destroyed)
- 3′ cloning site AttR2 (unknown if destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMT-GFP-Spindly was a gift from Ron Vale (Addgene plasmid # 24282 ; http://n2t.net/addgene:24282 ; RRID:Addgene_24282) -
For your References section:
Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore. Griffis ER, Stuurman N, Vale RD. J Cell Biol. 2007 Jun 18. 177(6):1005-15. 10.1083/jcb.200702062 PubMed 17576797