pI12
(Plasmid #24273)

• Depositing Lab: Mariano Garcia-Blanco
• Publication: Baraniak et al Mol Cell Biol. 2003 Dec . 23(24):9327-37.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5409
• Vector type: Mammalian Expression ; T7 for in vitro transcription
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: modified adenovirus exons and introns
• Alt name: splicing reporter
• Species: Modified from adenovirus
• Insert Size (bp): 246

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is heavily modified. Use our sequence and map rather than on based on pcDNA3. For example, HindIII and EcoRI are switched from pcDNA3.