pGSTag Bmf
(Plasmid
#24264)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24264 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGSTag
- Backbone size w/o insert (bp) 5057
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBmf
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Alt nameBcl2 modifying factor
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SpeciesH. sapiens (human)
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Insert Size (bp)590
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GenBank IDNM_001003940
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Entrez GeneBMF
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Tag
/ Fusion Protein
- Gst (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site xho 1 (not destroyed)
- 5′ sequencing primer pGex 5'
- 3′ sequencing primer pGex 3' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGSTag Bmf was a gift from Roger Davis (Addgene plasmid # 24264 ; http://n2t.net/addgene:24264 ; RRID:Addgene_24264) -
For your References section:
JNK phosphorylation of Bim-related members of the Bcl2 family induces Bax-dependent apoptosis. Lei K, Davis RJ. Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7. 10.1073/pnas.0438011100 PubMed 12591950