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Addgene

pGSTag BimL (S44A)
(Plasmid #24252)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24252 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGSTag
  • Backbone size w/o insert (bp) 5057
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BimL(S44A)
  • Alt name
    BCL2-like 11
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    436
  • Mutation
    Changed Serine 44 to Alanine
  • Entrez Gene
    BCL2L11 (a.k.a. BAM, BIM, BOD)
  • Tag / Fusion Protein
    • Gst (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (not destroyed)
  • 3′ cloning site xho 1 (not destroyed)
  • 5′ sequencing primer pGex 5'
  • 3′ sequencing primer pGex 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGSTag BimL (S44A) was a gift from Roger Davis (Addgene plasmid # 24252 ; http://n2t.net/addgene:24252 ; RRID:Addgene_24252)

  • For your References section:

    JNK phosphorylation of Bim-related members of the Bcl2 family induces Bax-dependent apoptosis. Lei K, Davis RJ. Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7. 10.1073/pnas.0438011100 PubMed 12591950
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