pCMVHA SUZ12
(Plasmid #24232)

• Purpose: Mammalian expression of human SUZ12 with HA tag
• Depositing Lab: Kristian Helin
• Publication: Pasini et al EMBO J. 2004 Oct 13. 23(20):4061-71.

Backbone

• Vector backbone: pCMVHANeoBam
• Backbone size w/o insert (bp): 6500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SUZ12
• Species: H. sapiens (human)
• Entrez Gene: SUZ12 (a.k.a. CHET9, IMMAS, JJAZ1)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Bglob-intron-F (5'-ctggtcatcatcctgccttt-3')
• 3′ sequencing primer: Bglob-pA-R (5'-ttttggcagagggaaaaaga-3')

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry