pCMVHA EED wt
(Plasmid #24231)

• Purpose: Mammalian expression of human EED with HA tag
• Depositing Lab: Kristian Helin
• Publication: Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

Backbone

• Vector backbone: pCMVHA
• Backbone size w/o insert (bp): 6600
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EED
• Species: H. sapiens (human)
• Entrez Gene: EED (a.k.a. COGIS, HEED, WAIT1)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: Bglob-intron-F (5'-ctggtcatcatcctgccttt-3')
• 3′ sequencing primer: Bglob-pA-R 5'-ttttggcagagggaaaaaga-3'

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry