pCMVHA E2F2
(Plasmid #24226)

• Purpose: Mammalian expression of human E2F2 with HA tag
• Depositing Lab: Kristian Helin
• Publication: Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

Backbone

• Vector backbone: pCMVHANeoBam
• Backbone size w/o insert (bp): 6600
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E2F2
• Alt name: E2F-2
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Entrez Gene: E2F2 (a.k.a. E2F-2)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: Bglob-intron-F
• 3′ sequencing primer: Bglob-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert check with SalI, which leads to three fragments 4.7kb 1,8kb 0,35kb. E2F1 cDNA can be removed by BamHI digest.