pCMVHA E2F1
(Plasmid #24225)

• Purpose: Mammalian expression of human E2F1 with HA tag
• Depositing Lab: Kristian Helin
• Publication: Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

Backbone

• Vector backbone: pCMVHANeoBam
• Backbone size w/o insert (bp): 6200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E2F1
• Alt name: E2F-1
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Entrez Gene: E2F1 (a.k.a. E2F-1, RBAP1, RBBP3, RBP3)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: Bglob-intron-F
• 3′ sequencing primer: Bglob-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 26 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert check with SalI, which leads to a 1.3kb fragment + vector. E2F1 cDNA can be removed by BamHI digest.

This plasmid contains an E356K mutation within the E2F1 insert. Please note that other mutations may exist and it is unclear whether this mutation affects plasmid function.