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Addgene

pRint
(Plasmid #24218)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24218 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA6/V5/His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5002
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RFP with modified adeno intron
  • Alt name
    RFP splicing reporter
  • Species
    synthetic and adenovirus
  • Insert Size (bp)
    815
  • Mutation
    dsRED interrupted by a modified adenovirus intron

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRint was a gift from Mariano Garcia-Blanco (Addgene plasmid # 24218 ; http://n2t.net/addgene:24218 ; RRID:Addgene_24218)

  • For your References section:

    A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice. Bonano VI, Oltean S, Garcia-Blanco MA. Nat Protoc. 2007 . 2(9):2166-81. 10.1038/nprot.2007.292 PubMed 17853873
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