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Addgene

pGint
(Plasmid #24217)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24217 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3945
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pGint
  • Alt name
    GFP splicing reporter
  • Species
    Modified from Aequorea victoria and adenovirus
  • Insert Size (bp)
    859
  • Mutation
    Modified adenovirus in GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer commercial, from Clontech
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGint was a gift from Mariano Garcia-Blanco (Addgene plasmid # 24217 ; http://n2t.net/addgene:24217 ; RRID:Addgene_24217)

  • For your References section:

    A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice. Bonano VI, Oltean S, Garcia-Blanco MA. Nat Protoc. 2007 . 2(9):2166-81. 10.1038/nprot.2007.292 PubMed 17853873
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