pCMV E2F1 1-284
(Plasmid #24216)

• Purpose: Mammalian expression of human E2F1 (aa 1-284) with HA tag
• Depositing Lab: Kristian Helin
• Publication: Helin et al Genes Dev. 1993 Oct . 7(10):1850-61.

Backbone

• Vector backbone: pCMVNeoBam
• Backbone size w/o insert (bp): 6600
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E2F1 1-284
• Alt name: E2F-1 1-284
• Species: H. sapiens (human)
• Insert Size (bp): 850
• Entrez Gene: E2F1 (a.k.a. E2F-1, RBAP1, RBBP3, RBP3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: Bglob-intron-F
• 3′ sequencing primer: Bglob-pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NB! No in frame stop codon inserted just after the aa284, i.e translation stops downstream in the poly A sequence from betaglobin